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AssigneesInstitut Pasteur, Institut National de la Sante et de la Recherche Medicale
InventorsPierre Charneau, Veronique Zennou, Francoise Pflumio, Aride Sirven, Anne Dubart
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The present invention provides nucleic acid, vectors, viruses, and recombinant cells comprising triple-stranded structures, such as those resulting from central initiation and termination of HIV-1 reverse transcription at the center of HIV-1 linear DNA genomes. These triplex structures can act as a cis-determinant of HIV-1 DNA nuclear import, allowing infection of non-dividing target cells. In one aspect, the presence of the DNA triplex sequence in an HIV vector strongly stimulates gene transfer in hematopoietic stem cells. The invention also provides methods of using these triplex structures for making recombinant cells, as well as methods of using the recombinant cells to express proteins of interest both in vitro and in vivo.
An isolated nucleic acid comprising the nucleic acid sequence of the EcoRI/MluI insert of vector pTRIP ΔU3 EF1αGFP in which the promoter and enhancer of the U3 sequence have been deleted, deposited at National Collection of Cultures of Microorganismes, Accession Number I-2328.
An isolated nucleic acid consisting of the nucleic acid sequence of the EcoRI/MluI insert of vector pTRIP ΔU3 EF1αGFP in which the promoter and enhancer of the U3 sequence have been deleted, deposited at National Collection of Cultures of Microorganismes, Accession Number I-2328.
An isolated vector that is pTRIP ΔU3 EF1αGFP deposited at National Collection of Cultures of Microorganismes, Accession Number I-2328.
An isolated nucleic acid consisting of a nucleic acid sequence of the EcoRI/MluI insert of vector pTRIP ΔU3 EF1αGFP deposited at National Collection of Cultures of Microorganismes, Accession Number I-2328.
An isolated HIV-1 expression vector comprising:
An isolated HIV-1 expression vector comprising:
Dependencies are inferred from the claim text.