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US 8,093,014 B2Granted
Kit for detecting and measuring element tagged kinases and phosphatases by inductively coupled plasma mass spectrometry
AssigneeDvs Sciences Inc.
InventorOlga Ornatsky
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Abstract
Kits for the assay of enzymes involved in post-translational modifications are provided. The kits facilitate the execution of methods of assay that allow for convenient and accurate analysis using atomic mass spectrometry of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enzymes.
Claims15
A kit for detecting and measuring a mass-to-charge ratio of an element tag or a metal ion of a metal ion coordination complex by inductively coupled plasma mass spectrometry in a sample, comprising:
- (a) the element tag comprising a transition element, or a transition element isotope;
- (b) a non-phosphorylated substrate;
- (c) a support;
- (d) the metal ion coordination complex;
- (e) optionally, a kinase;
- (f) optionally, a kinase buffer;
- (g) and optionally, ATP;
- wherein the element tag is for directly tagging the support or the non-phosphorylated substrate.
5 more elements
Dependencies are inferred from the claim text.