Bis-modified bicyclic nucleic acid analogs

An oligomeric compound having the formula:

• G-[T-L]j-[R-L]q-R-[L-T]r-E
• wherein:
• each T is a monomer of Formula V:
• wherein independently for each monomer having Formula V:
• Bx is a heterocyclic base moiety;
• one of Z1 and Z2 is H, C1-C6 alkyl, substituted C1-C6 alkyl, C2-C6 alkenyl, substituted C2-C6 alkenyl, C2-C6 alkynyl or substituted C2-C6 alkynyl and the other one of Z1 and Z2 is C1-C6 alkyl, substituted C1-C6 alkyl, C2-C6 alkenyl, substituted C2-C6 alkenyl, C2-C6 alkynyl or substituted C2-C6 alkynyl;
• one of Q1 and Q2 is H, C1-C6 alkyl, substituted C1-C6 alkyl, C2-C6 alkenyl, substituted C2-C6 alkenyl, C2-C6 alkynyl, substituted C2-C6 alkynyl, thiol or substituted thiol and the other one of Q1 and Q2 is C1-C6 alkyl, substituted C1-C6 alkyl, C2-C6 alkenyl, substituted C2-C6 alkenyl, C2-C6 alkynyl, substituted C2-C6 alkynyl, thiol or substituted thiol;
• or Q1 and Q2 together are methylene (═CH2);
• each R is a β-D-2′-deoxyribonucleoside;
• each L is an internucleoside linking group;
• G and E are each, independently, H, a hydroxyl protecting group, a linked conjugate group or a 5′ or 3′-terminal group;
• wherein each substituted group is mono or poly substituted with substituent groups independently selected from halogen, OJ1, SJ1, NJ1J2, N3, COOJ1, CN, O—C(═O)NJ1J2, N(H)C(═NH)NJ1J2, N(H)C(═O)N(H)J2 or N(H)C(═S)N(H)J2;
• each J1 and J2 is, independently, H, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C1-C6 aminoalkyl or a protecting group;
• q is from 7 to about 15;
• j is 0 or from 1 to about 5;
• r is 0 or from 1 to about 5;
• and
• the sum of j and r is at least 1.