Thrombin mutant
AssigneesChisso Corporation, Fujimori Kogyo Co., Ltd.
InventorsKazuya Hosokawa, Tomoko Wada
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Abstract
A thrombin mutant in which at least serine at position 205 among amino acids in the active center of the thrombin B chain has been replaced with another amino acid, and further at least one of the following replacements have been introduced: (I) replacement of arginine at position 89 in the B-chain with another amino acid; (II) replacement of threonine at position 69 or serine at position 22 in the B-chain with another amino acid; (III) replacement of alanine at position 200 in the B-chain with another amino acid; and (IV) replacement of lysine at position 65 in the B-chain with threonine.
Claims16
A thrombin mutant comprising:
- an isolated B chain that comprises a replacement of a serine at position 205 and
- a threonine at position 69 in the isolated B chain with another amino acid, wherein the isolated B chain is counted from amino acid number 50 of SEQ ID NO:2, and wherein the thrombin mutant comprises an activated partial thromboplastin time (APTT) prolonging effect.
Dependencies are inferred from the claim text.