Reverse genetic system for rift valley fever virus and uses thereof
AssigneeBoard of Regents, The University of Texas System
InventorsShinji Makino, Tetsuro Ikegami, Clarence J. Peters, Sungyong Won
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Abstract
The present invention describes a reverse genetic system for Phlebovirus such as Rift Valley fever virus. This system comprised of RNA expression plasmids and protein expression plasmids. Additionally, the present invention also discloses the modification of this system to generate a recombinant virus that expresses a non-viral foreign gene. Furthermore, the present invention discloses the use of this system in the development of anti-Rift Valley fever virus vaccines, screening of antivirals testing for anti RVF immune response and developing marker vaccines for Rift Valley fever virus. We also claim the utility of this approach to other phleboviruses.
Claims10
A reverse genetic composition for a Phlebovirus, comprising:
- at least one anti-viral sense RNA expression plasmid that encodes a non-viral gene inserted in place of a non-structural protein (NSs) open reading frame (ORF) in the Phlebovirus Small (S) segment, such that said non-structural protein (NSs) open reading frame (ORF) for said Phlebovirus is deleted but the N gene is not deleted;
- at least one anti-viral sense RNA expression plasmid that encodes a Phlebovirus Large (L) segment;
- at least one anti-viral sense RNA expression plasmid that encodes a Phlebovirus Medium (M) segment;
- at least one viral protein expression plasmid that expresses a Phlebovirus L protein;
- at least one viral protein expression plasmid that expresses a Phlebovirus N protein;
- and
- at least one viral protein expression plasmid that expresses Phlebovirus envelope proteins.
Dependencies are inferred from the claim text.