# US 8,084,037 B2 · Recombinant parainfluenza virus expression systems and vaccines

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- Number: US 8,084,037 B2
- Document ID: US8084037B2
- Status: Granted
- Kind: B2
- Application number: 12/694,366
- Filed: 2010-01-27
- Published: 2010-05-13
- Granted: 2011-12-27
- Earliest claimed: 2001-03-21
- Assignees: MedImmune, LLC
- Inventors: Aurelia Haller; Kathleen Coelingh
- Web page: https://patentgrep.com/patent/US8084037B2

## CPC

- C12N 15/86: Viral vectors
- A61K 39/12: Viral antigens
- A61K 39/155: Paramyxoviridae, e.g. parainfluenza virus
- A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P 31/14: for RNA viruses
- A61P 31/16: for influenza or rhinoviruses
- A61P 37/04: Immunostimulants
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 2039/5256: {expressing foreign proteins}
- A61K 2039/53: {DNA (RNA) vaccination}
- C12N 2760/18621: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2760/18634: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2760/18643: viral genome or elements thereof as genetic vector
- C12N 2760/18661: Methods of inactivation or attenuation
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to recombinant bovine parainfluenza virus (bPIV) cDNA or RNA which may be used to express heterologous gene products in appropriate host cell systems and/or to rescue negative strand RNA recombinant viruses that express, package, and/or present the heterologous gene product. The chimeric viruses and expression products may advantageously be used in vaccine formulations including vaccines against a broad range of pathogens and antigens.

## Claims

### Claim 1

An immunogenic composition comprising a chimeric Kansas-strain bovine parainfluenza virus type 3 (BPIV3), said chimeric BPIV3 comprising:
(i) a Kansas-strain BPIV3 genome, and
(ii) a heterologous sequence encoding an immunogenic epitope from a virus,
wherein said heterologous sequence is inserted into said Kansas-strain BPIV3 genome at nucleotide position 5041, nucleotide position 8529, or within the hemagglutinin-neuraminidase glycoprotein gene.

### Claim 2 (depends on 1)

The immunogenic composition of claim 1, wherein the immunogenic epitope is from a respiratory syncytial virus glycoprotein.

### Claim 3

An immunogenic composition comprising a chimeric Kansas-strain BPIV3, said chimeric BPIV3 comprising:
(i) nucleotides 1-5041 and nucleotides 8529-15456 of a Kansas-strain BPIV3 genome; and
(ii) a heterologous sequence encoding an immunogenic epitope from a virus.

### Claim 4 (depends on 3)

The immunogenic composition of claim 3, wherein the immunogenic epitope is from a respiratory syncytial virus glycoprotein.