System and method of capturing multiple source excitations from a single location on a flow channel
AssigneeAccuri Cytometers, Inc.
InventorsDavid C. Olson, Collin A. Rich
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Abstract
The invention includes a system and a method for capturing multi source excitations from a single location on a flow channel. The system preferably includes a light subsystem that emits light onto a single location on a flow channel, a detector subsystem to detect light emitted from the single location on the flow channel, and a processor to separate the detected light. The method preferably includes emitting light onto a single location on a flow channel, detecting light emitted from the single location on the flow channel, and separating the detected light.
Claims18
A system for capturing multi source excitations from a single location on a flow channel comprising:
- a light subsystem that emits light onto a single location on a flow channel, wherein the light subsystem includes a first excitation source that emits light at a first wavelength and a second excitation source that emits light at a second wavelength that is different from the first wavelength, wherein the light subsystem modulates between the following excitation source operating modes:
- a first excitation source operating mode, wherein only one of the first and second excitation sources emits light onto the single location on the flow channel;
- and
- a second excitation source operating mode, wherein both the first and second excitation sources emit light onto the single location on the flow channel;
- a detector subsystem to that detects light emitted from the single location on the flow channel and generates a composite signal;
- and
- a processor coupled to the detector subsystem and adapted to separate the composite signal into composite mode signals each corresponding to a respective excitation source operating mode, wherein the processor is adapted to separate a composite mode signal into component mode signals corresponding to at least two overlapping emission spectra of fluorophores.
A method for capturing multi source excitations from a single location on a flow channel comprising the steps of:
- emitting light onto a single location on a flow channel, including emitting light from a first excitation source at a first wavelength and emitting light from a second excitation source at a second wavelength that is different from the first wavelength, wherein the step of emitting light includes modulating between the following excitation source operating modes:
- in a first excitation source operating mode, emitting light onto the single location on the flow channel from only one of the first and second excitation sources;
- and
- in a second excitation source operating mode, emitting light onto the single location on the flow channel from both the first and second excitation sources;
- detecting light emitted from the single location on the flow channel and generating a composite signal;
- separating the composite signal into two or more composite mode signals, wherein each composite mode signal corresponds to a respective excitation source operating mode, and
- separating one or more of the composite mode signals into component mode signals corresponding to at least two overlapping emission spectra of fluorophores.
Dependencies are inferred from the claim text.