Method of breeding germinable transgenic broadleaved tree species

Abstract

Methods of breeding germinable transgenic broadleaved tree species are disclosed which include the following steps: (1) the main stem of a tree is cutoff at the height of 50-160 cm above the ground; (2) the transection is dipped with 0.3-0.5 OD600 of a suspension of recombinant Agrobacterium containing exogenous target genes for 3 minutes to 24 hours; (3) selective breeding is performed on the third day after infection by dipping the transection infected by recombinant Agrobacterium in step 2 with antibiotics having lethal effect on the Agrobacterium every other 1-2 days to obtain transgenic regeneration plants. In the practical application, the method will have the following active effects: (1) it accelerates the transfer of good exogenous genes to germinable tree species, particularly poplar and facilitates the improvement of varieties thereof; (2) it provides a new way of asexual propagation of germinable broadleaved tree species and increases the productivity and the level of intensive cultivation of germinable broadleaved tree species, so it can be used to solve the ecological problems such as vegetation restoration and to meet the requirement of the market for wood.

Dependencies are inferred from the claim text.