Chlamydomonas glucan dikinase gene, enzyme and modified starch, uses, methods for production thereof
AssigneeSyngenta Participations AG
InventorsShib Sankar Basu, Michael B. Lanahan, Mark Kinkema
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Abstract
The present invention relates the nucleotide sequence from Chlamydomonas reinhardtii encoding a glucan dikinase enzyme and to methods of use; to modified starch, as well as production and uses thereof. The starch has modified properties of viscosity and a modified phosphate content.
Claims19
An isolated nucleic acid molecule having 95%, sequence similarity to SEQ ID NO:
- 26, wherein the nucleic acid encodes a polypeptide having glucan dikinase activity.
An isolated nucleic acid molecule encoding a polypeptide having 95% sequence similarity to SEQ ID NO:
- 27, wherein the polypeptide has glucan dikinase activity.
An isolated nucleic acid molecule having 95% sequence similarity to SEQ ID NO:
- 28, wherein the nucleic acid encodes a polypeptide having glucan dikinase activity.
An isolated nucleic acid molecule encoding a polypeptide having 95% sequence similarity to SEQ ID NO:
- 30, wherein the polypeptide has glucan dikinase activity.
An isolated polypeptide having 95% sequence similarity to SEQ ID NOs:
- 27 or 30, wherein the polypeptide has glucan dikinase activity.
A method of increasing the fermentable starch in plant or plant parts comprising:
- a. inserting an expression cassette into a plant cell comprising a nucleic acid encoding an green alga glucan dikinase polypeptide;
- b. regeneration of transgenic plants from the plant cell of a);
- and
- c. producing said fermentable starch.
A method of producing a modified phosphorylated starch in plant comprising:
- a. inserting an expression cassette into a plant cell comprising a nucleic acid encoding an green alga glucan dikinase polypeptide;
- b. regeneration of transgenic plants from the plant cell of a);
- and
- c. producing said modified starch.
Dependencies are inferred from the claim text.