Transgenic animal with monocyte chemotactic protein 1 promoter
AssigneeNational Health Research Institutes
InventorKurt Ming-Chao Lin
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Abstract
The present invention relates to a transgenic animal, which comprises in its genome a recombinant polynucleotide encoding one or more reporter proteins and a monocyte chemotactic protein-1 (MCP-1) promoter, wherein the one or more reporter proteins are expressed under the control of the MCP-1 promoter. A method for monitoring endogenous expression of MCP-1 in vivo is also provided, which is useful for identifying a regulator of the expression of MCP-1 or an anti-inflammatory agent.
Claims9
A non-human transgenic animal, which comprises in its genome a recombinant nucleic acid molecule comprising a monocyte chemotactic protein-1 (MCP-1) promoter and a polynucleotide encoding an enhanced GFP (EGFP) expressed under the control of the MCP-1 promoter, wherein the recombinant nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:
- 2.
A method for monitoring endogenous expression of MCP-1 in vivo comprising:
- (a) providing a non-human transgenic animal, which comprises in its genome a recombinant nucleic acid molecule comprising a monocyte chemotactic protein-1 (MCP-1) promoter and a polynucleotide encoding an EGFP expressed under the control of the MCP-1 promoter, and
- (b) detecting in the transgenic animal the presence or level of the EGFP as an indicator for the expression of the endogenous MCP-1;
- wherein the recombinant nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.