Method for producing antithrombin composition
AssigneeKyowa Hakko Kirin Co., Ltd.
InventorsTsutomu Sugihara, Setsuko Onodera, Tomonari Urakubo, Toshiyuki Suzawa
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Abstract
The provision of an antithrombin composition having a desired α-form content rate or β-form content rate is required. The invention provides a process for producing an antithrombin composition having a desired α-form content rate or β-form content rate which is prepared by contacting an antithrombin-containing aqueous solution with a Cellufine Sulfate chromatography carrier.
Claims14
A process for producing an antithrombin composition, comprising
- contacting an antithrombin-containing aqueous solution with a cellulose sulfate ester chromatography carrier to obtain an antithrombin composition comprising antithrombin with an α-form content rate of at least 80%;
- and
- eluting the antithrombin compositions from a fraction adsorbed onto the carrier with an eluent having a conductivity of 0.01 mS/cm to 50 mS/cm to obtain an eluate with the antithrombin composition.
A process for producing an antithrombin composition, comprising contacting a conductivity-adjusted antithrombin-containing aqueous solution with a cellulose sulfate ester chromatography carrier to obtain an antithrombin composition comprising antithrombin with an α-form content rate of at least 80% from a fraction unadsorbed onto the carrier, and
- collecting the antithrombin composition from the fraction unadsorbed onto the carrier,
- wherein the conductivity-adjusted antithrombin-containing aqueous solution has a conductivity of 0.1 mS/cm to 50 mS/cm.
Dependencies are inferred from the claim text.