Method of providing particles having biological-binding areas for biological applications
AssigneeLife Bioscience, Inc.
InventorsJeb H. Flemming, Colin T. Buckley, Carrie Schmidt
Life of this patent
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Abstract
The present invention includes micro-sphere composition, methods of making binding assays. The present invention also includes a micro-sphere for binding biological molecules without pretreatment. The micro-sphere includes a spherical glass substrate having one or more metal nanoparticle regions that are exposed from within the glass, wherein the micro-sphere is capable of binding biological molecules without pretreatment.
Claims24
A method of providing a bead having discrete binding areas for biological-testing, comprising:
- preparing a glass bead substrate by at least one process step selected from the group consisting of
- exposing the glass bead to an activating energy source, baking the glass bead having a glass transition temperature at a temperature above the glass transition temperature, etching at least one selected area of the glass bead to expose metal nanoparticles from within the glass, wherein the metal nanoparticles have a range of diameters and are spaced from other metal nanoparticles by an average distance of at least one-half the midpoint of the diameter range, and the metal nanoparticles are less than about 300 nanometers in diameter, whereby the at least one selected area provides a chemistry for increased binding as compared to unselected areas.
A substrate for binding biological molecules without pretreatment comprising:
- an at least partially spherical glass substrate comprising one or more metal particle regions that are exposed from within the at least partially spherical glass substrate, wherein the substrate is capable of binding biological molecules without pretreatment.
A micro-sphere binding assay for binding biological molecules without pretreatment comprising:
- preparing a glass micro-sphere bead substrate by least one process step selected from the group consisting of exposing the glass micro-sphere bead to an activating energy source, baking the glass micro-sphere bead having a glass transition temperature at a temperature above the glass transition temperature, etching at least one selected area of the glass micro-sphere bead to expose metal nanoparticles from within the glass micro-sphere bead;
- and depositing patterned areas of biological-binding-glass on a substrate, wherein the metal nanoparticles have a range of diameters and are spaced from other metal nanoparticles by an average distance of at least one-half the midpoint of the diameter range, and the metal nanoparticles are less than about 300 nanometers in diameter, whereby the at least one selected area provides a chemistry for increased binding as compared to unselected areas;
- binding one or more biological molecules to the glass micro-sphere bead;
- and
- detecting the presence of the one or more biological molecules.
Dependencies are inferred from the claim text.