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US 8,076,148 B2Granted
Cytochrome c protein and assay
AssigneeGe Healthcare UK Limited
InventorsSimon L. Stubbs, Michael J. Francis, Adrian Cushing, Rahman A. Ismail
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Abstract
The present invention relates to a cytochrome c-reporter fusion protein construct comprising a modified cytochrome c protein which targets the mitochondria and has a reduced ability to induce apoptosis in a living cell. The invention also relates to nucleic acid constructs encoding such protein fusions and cells stably transfected with such constructs. The stably transfected cells of the invention can be used in assays to detect apoptosis.
Claims29
A cytochrome C-reporter fusion protein construct comprising:
- (a) a modified cytochrome C protein derived from wild type human cytochrome C represented by SEQ ID NO:
- 2;
- and
- (b) a fluorescent protein,
- wherein said fusion protein targets the mitochondria and has a reduced ability to induce apoptosis in a living cell, and
- wherein said modified cytochrome C comprises the amino acid substitution selected from the group consisting of K73A, K73L, K73R, K73G and K73X, wherein X represents trimethylation.
3 more elements
A nucleotide sequence selected from the group consisting of SEQ ID NO:
- 3 and SEQ ID NO 5.
Dependencies are inferred from the claim text.