Method and apparatus for analyzing vitamin E in lipoproteins
AssigneeTosoh Corporation
InventorYuji Hirowatari
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Abstract
A method of analyzing vitamin E components in a lipoprotein by subjecting a lipoprotein-containing sample to ion exchange chromatography to separate the lipoprotein, reacting the separated lipoprotein to a pretreating solution containing an organic solvent and a surfactant to liberate vitamin E components, and then subjecting the liberated vitamin E components to reverse phase chromatography. Also described is a method of judging various pathological conditions such as the pathological conditions of diabetes, the risks of coronary artery diseases, and the pathological conditions of myocardial infarction using levels of vitamin E components in the lipoprotein as an index.
Claims3
A method of analyzing vitamin E components in sample containing lipoproteins, which comprises
- subjecting the sample to an ion exchange chromatography to separate the lipoproteins from the sample,
- reacting the separated lipoproteins with a pretreating solution containing an organic solvent and a surfactant to liberate vitamin E components from the separated lipoproteins, and then
- subjecting the liberated vitamin E components to a reverse phase chromatography.
Dependencies are inferred from the claim text.