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US 8,076,142 B2Granted
Rooted plant assay system
AssigneeBASF Plant Sciences GmbH
InventorsXiang Huang, Ming Cheng, Haiping Hong, Yan Liu, Kangfeng Mei
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Abstract
Disclosed herein are rooted plant assay systems, methods of making rooted plant assay systems, and methods of using rooted plant assay systems for high throughput screening of test polynucleotides for their ability to effect phenotypic traits having agricultural value.
Claims4
A method of making a rooted plant assay system consisting of the steps of:
- a) obtaining a plant tissue explant;
- b) infecting said explant with disarmed Agrobacterium rhizogenes comprising transgenic T-DNA, wherein said T-DNA comprises a selectable marker gene and a test polynucleotide,
- c) growing the infected explant on a medium comprising:
- i) an effective amount of at least one root-inducing auxin compound, and
- ii) an effective amount of a selection agent;
- and
- d) selecting transgenic non-hairy roots that grow on said medium.
4 more elements
A method of making a rooted plant assay system consisting of the steps of:
- e) obtaining a wounded dicot tissue explant;
- f) adding the explant to a disarmed Agrobacterium tumnefaciens comprising a transgenic T-DNA, wherein said T-DNA comprises a selectable marker gene and a test polynucleotide, in a co-cultivation medium comprising a cytokinin obtain an infected explant;
- g) growing said infected explant on a selection medium comprising:
- i) an effective amount of a root-inducing auxin compound, and
- ii) an effective amount of a selection agent;
- and
- h) selecting transgenic non-hairy roots that grow on said selection medium.
4 more elements
Dependencies are inferred from the claim text.