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US 8,076,120 B2Granted
3-hydroxypropionic acid and other organic compounds
AssigneeCargill, Incorporated
InventorsRavi R. Gokarn, Olga V. Selifonova, Holly Jean Jessen, Steven John Gort, Thorsten Selmer, Wolfgang Buckel
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Abstract
Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.
Claims11
An isolated transformed cell comprising:
- a nucleic acid molecule comprising at least 90% sequence identity to SEQ ID NO:
- 9, encoding an E1 activator of an enzyme having lactyl-CoA dehydratase activity;
- a nucleic acid molecule comprising at least 90% sequence identity to SEQ ID NO:
- 17, encoding an E2 α subunit of an enzyme having lactyl-CoA dehydratase activity;
- a nucleic acid molecule comprising at least 90% sequence identity to SEQ ID NO:
- 25, encoding an E2 β subunit of an enzyme having lactyl-CoA dehydratase activity;
- a nucleic acid molecule comprising at least 90% sequence identity to SEQ ID NO:
- 40, encoding an enzyme having 3-hydroxypropionyl-CoA dehydratase activity;
- and
- a nucleic acid molecule encoding an enzyme having poly hydroxyacid synthase activity.
7 more elements
Dependencies are inferred from the claim text.