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US 8,076,111 B2Granted
Method for producing an organic acid
AssigneeAjinomoto Co., Inc.
InventorsKeita Fukui, Yoshinori Tajima, Kazue Kawamura, Yoshihiro Usuda, Kazuhiko Matsui
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Abstract
An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of yidE gene is enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.
Claims9
A method for producing succinic acid comprising:
- A) allowing a substance to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas, wherein the substance is selected from the group consisting of:
- i) a bacterium which has an ability to produce an organic acid and has been modified to have enhanced expression of the yidE gene,
- ii) a product obtained by processing the bacterium of i),
- iii) combinations thereof, and
- B) collecting the succinic acid:
- wherein the yidE gene is selected from the group consisting of:
- (a) a DNA comprising the nucleotide sequence of SEQ ID NO:
- 46, and
- (b) a DNA which hybridizes with a DNA comprising a nucleotide sequence complementary to the entire nucleotide sequence of SEQ ID NO:
- 46 under stringent conditions comprising washing with 1×SSC, 0.1% SDS at 60° C., and said DNA improves the ability of the bacterium to produce an organic acid when expression of the DNA is enhanced in the bacterium;
- wherein the enhanced expression is obtained by a method selected from the group consisting of:
- i) increasing the copy number of the yidE gene,
- ii) replacing a promoter sequence of the yidE gene with a stronger promoter, and
- iii) combinations thereof.
11 more elements
Dependencies are inferred from the claim text.