Biomarkers of Francisella infection
AssigneeBoard of Regents, The University of Texas System
InventorsJames Chambers, Jieh-Juen Yu, Karl E. Klose, M. Neal Guentzel, Bernard P. Arulanandam
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Abstract
The instant invention concerns Francisella bacteria mediated degradation of alkaline phosphatase (AP). Detection of AP degradation may be used to determine the presence of Francisella bacteria in a sample. Furthermore, methods for identifying and treating Francisella infections by detecting AP degradation are described. Methods of the invention also concerns methods for treating Francisella infection by inhibiting AP degradation.
Claims68
A method for determining whether a subject has a Francisella infection comprising detecting alkaline phosphatase (AP) degradation activity in a sample from the subject by detecting degradation of a substrate added to the sample, wherein AP degradation activity is indicative of Francisella infection.
A method for determining whether a subject has a Francisella infection comprising detecting alkaline phosphatase (AP) degradation activity in a sample from the subject by detecting an AP degradation product in the sample, wherein AP degradation activity is indicative of Francisella infection.
A method for assisting in determining whether a subject has a Francisella infection, comprising:
- a) detecting AP degradation activity in a sample from the subject;
- and
- b) reporting information regarding AP degradation activity in the sample.
A method for treating an infection in a subject comprising:
- a) obtaining or having a sample from a subject;
- b) detecting AP degradation activity in the sample, wherein AP degradation activity is indicative Francisella infection;
- and
- c) treating the subject with an antibiotic that is effective against Francisella infection or another drug depending on whether AP degradation activity is detected.
A method for determining the presence of Francisella bacteria in a sample comprising detecting AP degradation activity in the sample by detecting degradation of a substrate added to the sample.
A method for determining the presence of Francisella bacteria in a sample comprising detecting AP degradation activity in the sample by detecting an AP degradation product in the sample.
A method for determining the efficacy of a Francisella vaccine composition in a subject comprising:
- a) administering a Francisella vaccine composition to a subject;
- b) administering a Francisella bacteria to the subject wherein the Francisella bacteria is capable of reducing AP activity in the serum of an unvaccinated subject;
- c) obtaining a sample from the subject;
- and
- d) determining the amount of AP degradation activity in the sample, thereby determining the efficacy of the vaccine composition.
Dependencies are inferred from the claim text.