Gene encoding a multidrug-resistance human P-glycoprotein homologue on chromosome 7P15-21 and uses thereof
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsMarkus H. Frank, Mohamed H. Sayegh
Life of this patent
Estimating the term…
Abstract
The invention relates to an MDR family P-glycoprotein located on human chromosome 7p15-21, polynucleotide sequences encoding this P-glycoprotein and fragments thereof. This gene is utilized in methods for assessing cancer cell susceptibility to therapies directed against multidrug resistance, and for the design of diagnostic and therapeutic methods relating to cancer multidrug resistance. The invention also relates to methods for determining whether a test compound may inhibit multidrug resistance.
Claims5
A method of enriching stem cells from a cell mixture, comprising
- contacting a cell mixture with an antibody directed against a 7p P-glycoprotein having a protein sequence selected from the group consisting of SEQ ID NO:1, NO:2, and NO:3 and isolating stem cells bound to the antibody from the cell mixture.
A method of obtaining 7p P-glycoprotein positive stem cells comprising:
- obtaining a sample from a human donor;
- and isolating 7p P-glycoprotein positive cells from the sample, wherein the 7p P-glycoprotein positive cells are isolated using immobilized antibody against 7p P-glycoprotein having a protein sequence selected from the group consisting of SEQ ID NO:1, NO:2, NO:3, NO:4, NO:5, NO:6 NO:7 and NO:8.
A method of characterizing a stem cell, comprising
- contacting a stem cell with an antibody directed against a 7p P-glycoprotein having a protein sequence selected from the group consisting of SEQ ID NO:1, NO:2, and NO:3, to determine if the stem cell is 7p P-glycoprotein positive.
Dependencies are inferred from the claim text.