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US 8,076,088 B2Granted
Method of detecting human β-defensins
AssigneeCase Western Reserve University
InventorsAaron Weinberg, Santosh K. Ghosh
Life of this patent
Estimating the term…
Abstract
A method of detecting β-defensin in a bodily sample from a subject includes reducing the electrostatic interaction between β-defensin and negatively charged moieties in the bodily sample prior to detecting the β-defensin with an antibody or epitope binding fragment thereof.
Claims20
A method of detecting β-defensin in a bodily sample of a subject comprising:
- obtaining a bodily sample from the subject;
- reducing the electrostatic interaction between β-defensin and negatively charged moieties in the bodily sample;
- contacting the bodily sample with a capture antibody or an epitope binding fragment thereof, the capture antibody or an epitope binding fragment thereof being immunoreactive with and binding to the β-defensin;
- and
- detecting bound β-defensin in the bodily sample.
2 more elements
A method of quantifying β-defensin in a bodily sample of a subject comprising:
- obtaining a bodily sample from the subject;
- reducing the electrostatic interaction between β-defensin and negatively charged moieties in the bodily sample;
- contacting the bodily sample with a capture antibody or an epitope binding fragment thereof, the capture antibody or an epitope binding fragment thereof being immunoreactive with the β-defensin;
- contacting the bound β-defensin with a detection antibody or epitope binding fragment thereof, the detection antibody or epitope binding fragment thereof being coupled to a detectable label;
- detecting the detectable label in the sample;
- and
- correlating the amount of detectable label in the sample to an amount of β-defensin in the bodily sample.
4 more elements
A method of quantifying β-defensin in a bodily sample from a subject comprising:
- obtaining a bodily sample from the subject;
- adding CaCl2 to the bodily sample at an amount effective to reduce electrostatic interaction between β-defensin and negatively charged moieties in the bodily sample
- contacting the bodily sample with a capture antibody or an epitope binding fragment thereof, the capture antibody or an epitope binding fragment thereof being immunoreactive with the β-defensin;
- contacting the bound β-defensin with a detection antibody or epitope binding fragment thereof, the detection antibody or epitope binding fragment thereof being coupled to a detectable label;
- detecting the detectable label in the bodily sample;
- and
- correlating the amount of detectable label in the sample to an amount of β-defensin in the bodily sample.
4 more elements
Dependencies are inferred from the claim text.