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AssigneeSysmex Corporation
InventorsSachiyo Tada, Yasumasa Akai, Yasuyuki Imura, Shigeki Abe, Harumi Minekawa
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It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.
A primer set for loop-mediated isothermal polymerase chain reaction to detect Cytokeratin 19, comprising first, second, third, and fourth primers, wherein the first primer onsists of the nucleotide sequence set forth in SEQ ID NO. 419, the second primer consists of the nucleotide sequence set forth in SEQ ID NO. 421, the third primer consists of the nucleotide sequence set forth in SEQ ID NO. 358, and the fourth primer consists of the nucleotide sequence complementary to SEQ ID NO. 379.
Dependencies are inferred from the claim text.