Probe-antiprobe compositions and methods for DNA or RNA detection
AssigneeGenetag Technology, Inc.
InventorDavid A. Shafer
Life of this patent
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Abstract
The invention provides novel compositions and methods for detecting unlabeled nucleic acid targets using labeled polynucleotide probes and partially complementary antiprobes. The interaction of probes, antiprobes and targets result in signaling changes that indicate target frequency. This novel detection mechanism is called a DNA detection switch, and it enable end-point detection, microarray detection and real-time PCR detection of a variety of nucleic acid targets including microbial species and subspecies, drug resistant mutants, and pathogenic strains.
Claims12
A universal probe-antiprobe composition to detect a target nucleotide sequence in a sample, the composition comprising:
- (a) a universal linker/primer comprising
- (i) a 5′ universal linker sequence, wherein the 5′ universal linker sequence is not complementary to any sequence in the sample;
- and
- (ii) a 3′ primer sequence complementary to the target nucleotide sequence;
- (b) a universal probe comprising
- (i) a universal primer sequence that substantially corresponds to the universal linker sequence of the universal linker/primer;
- and
- (ii) a first labeling component;
- and
- (c) a universal antiprobe comprising
- (i) a sequence complementary to a partial sequence of the universal probe;
- and
- (ii) a second labeling component;
- the composition suitable for real-time amplification and detection of a specific target sequence.
Dependencies are inferred from the claim text.