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US 8,076,064 B2Granted
Method of treatment of RNA sample
AssigneeAgilent Technologies, Inc.
InventorHui Wang
Life of this patent
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Abstract
The invention relates to methods for treating samples of RNA. In an embodiment the method includes contacting the sample of RNA with an analogous DNA set to provide a DNA/RNA duplex. The analogous DNA set includes at least one sequence analogous to a small RNA. The method includes contacting the DNA/RNA duplex with an enzyme having a DNA:RNA nuclease activity to provide a digested RNA sample. Kits in accordance with the invention are also described.
Claims18
A method of treating a sample of RNA, the method comprising:
- a) contacting the sample of RNA with an analogous DNA set to provide a DNA/RNA duplex, wherein the analogous DNA set comprises at least one DNA oligonucleotide comprising at least ten contiguous nucleotides of a small RNA in said sample, except that uracils in the sequence of said small RNA are substituted with thymines in said DNA oligonucleotide;
- b) contacting the DNA/RNA duplex with an enzyme having an RNase H activity to provide a digested RNA sample;
- and
- c) analyzing said digested RNA sample to detect said small RNA.
1 more element
Dependencies are inferred from the claim text.