US 8,076,062 B2 · Mutational profiles in HIV-1 protease correlated with phenotypic drug resistance

Abstract

The present invention is directed to the field of nucleic acid diagnostics and the identification of base variation in target nucleic acid sequences. More particularly, the present invention relates to the use of such genotypic characterization of a target population of HIV and the subsequent association, i.e., correlation, of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention also relates to methods of utilizing the mutational profiles of the invention in drug development, i.e., drug discovery, drug design, drug modification, and therapy, treatment design, clinical management and diagnostic analysis.

Claims6

Dependent claims

A method of evaluating the relative effectiveness of drugs against mutant HIV reverse transcriptase comprising at least one mutation T386A, comprising the steps of:

(i) providing a HIV reverse transcriptase comprising at least one mutation T386A, said position number is with reference to the HIV strain IIIB/LAI;
(ii) determining the activity of said drugs on said mutant HIV reverse transcriptase;
(iii) determining the activity of said drugs on a corresponding wild type HIV reverse transcriptase;
(iv) determining the ratio of the activity determined in step (iii) over the activity determined in step (ii) for each drug;
and
(v) evaluating the relative effectiveness of the drugs against said mutant HIV reverse transcriptase based on the ratio of step (iv).

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

12/261,475Filed 30 Oct 2008 · division

Priority

1 Jul 2002

Published

US 2009/0061420 A15 Mar 2009

Granted

13 Dec 2011

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

G01N 33/48
Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00: biological material, e.g. blood, urine ;…
C12Q 1/48
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
C12Q 1/703
For retroviruses: viruses associated with AIDS
G01N 2333/16
Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV: hiv-1, hiv-2

Family

Application60/393,005Us provisional application · filed 1 Jul 2002

PCT/EP03/50280Parent of this division · filed 30 Jun 2003

US 2009/0061420 A1Pre-grant publication · published 5 Mar 2009

Application10/518,525Parent of this division

US 7,473,524 B2Parent of this division

US 2005/0239053 A1Published application

US 8,076,062 B2This patent

From the US filing history. Foreign family members aren’t listed.

Mutational profiles in HIV-1 protease correlated with phenotypic drug resistance

Life of this patent

Mutational profiles in HIV-1 protease correlated with phenotypic drug resistance

Life of this patent

Abstract

Claims6