Microfluidic chips for rapid multiplex ELISA
AssigneeThe Ohio State University Research Foundation Bioprocessing Innovative Company
InventorsShang-Tian Yang, Yunling Bai, Wei-Cho Huang
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Abstract
A microfluidic chip comprises a substrate and a channel or multiple channels in the substrate. Each channel includes a tortuous path section or multiple tortuous path sections. A receptor for the detection of an analyte can be immobilized in a tortuous path section, for example by adsorption. Different receptors can be immobilized in different tortuous path sections of each channel or in different channels for simultaneous detection of multiple analytes. The chip is especially useful for running immunoassays, particularly ELISA.
Claims32
A microfluidic chip for detecting the presence of analytes in a sample fluid, comprising:
- a substrate;
- and
- a continuous channel in the substrate, the continuous channel being defined by a flowpath between an inlet and an outlet, the channel having a plurality of tortuous path sections, each tortuous path section having a different receptor immobilized on its surface, each receptor being complementary to one analyte being tested for.
A device for detecting the presence of an analyte in a sample fluid, comprising:
- a substrate;
- and a continuous channel in the substrate, the continuous channel comprising an inlet, an outlet, and a flowpath connecting the inlet and outlet, wherein the inlet and outlet together define a midplane;
- and
- wherein the channel has a plurality of tortuous path sections, each tortuous path section having a different receptor immobilized on its surface, each receptor being complementary to an analyte being tested for:
- wherein in each tortuous path section, a portion of the flowpath travels transversely across the midplane.
A method of using a microfluidic chip to analyze a sample for an analyte, comprising:
- providing a sample and a microfluidic chip, the chip comprising a substrate and a continuous channel in the substrate, the channel being defined by a flowpath between an inlet and an outlet, the flowpath containing a plurality of tortuous path sections;
- immobilizing a different receptor in each tortuous path section, one of the receptors being complementary to the analyte being tested for;
- and
- flowing the sample, a conjugate solution, and an enzyme substrate sequentially through the continuous channel to perform an enzyme-linked immunosorbent assay on the sample.
A method of using a microfluidic chip to analyze a sample for multiple analytes, comprising:
- providing a sample and a microfluidic chip, the chip comprising a substrate and a continuous channel in the substrate, the channel being defined by a flowpath between an inlet and an outlet, the flowpath containing a plurality of tortuous path sections;
- including a different receptor in each tortuous path section, each receptor being complementary to an analyte;
- and
- flowing the sample, a conjugate solution, and an enzyme substrate sequentially through the continuous channel to perform an enzyme-linked immunosorbent assay on the sample.
A method of using a microfluidic chip to analyze a sample for multiple analytes, comprising:
- providing a sample and a microfluidic chip, the chip comprising a substrate and a plurality of continuous channels in the substrate, each continuous channel being defined by a flowpath between an inlet and an outlet, the flowpath comprising a plurality of tortuous path sections;
- including a different receptor in each tortuous path section, each receptor being complementary to an analyte;
- and
- flowing a portion of the sample through each continuous channel to perform an enzyme-linked immunosorbent assay on the portion of the sample being flowed through the channel, the sample being loaded into each channel by dipping one end of the microfluidic chip into a solution containing the sample.
A method of using a microfluidic chip to simultaneously analyze multiple samples for multiple analytes, comprising:
- providing multiple samples and a microfluidic chip, the chip comprising a substrate and a plurality of continuous channels in the substrate, each continuous channel being defined by a flowpath between an inlet and an outlet, the flowpath including a plurality of tortuous path sections;
- immobilizing a receptor complementary to an analyte in each tortuous path section;
- and
- flowing a different sample through each continuous channel to simultaneously perform an enzyme-linked immunosorbent assay on each sample.
Dependencies are inferred from the claim text.