Polymeric sorbent sheets as ion reservoirs for electroblotting
AssigneeBio-Rad Laboratories, Inc.
InventorsXuemei Yang, Cory M. Panattoni
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Abstract
Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.
Claims22
A transfer stack for transfer of electrophoretically separated species from a gel to a membrane, said transfer stack comprising:
- said gel,
- said membrane immediately adjacent to said gel and in flat surface contact therewith, and
- two sheets of absorbent material of sufficient thickness to retain buffer solution, one said sheet immediately adjacent to said gel on the side thereof opposite the side in contact with said membrane and the other said sheet immediately adjacent to said membrane on the side thereof opposite the side in contact with said gel, each said sheet consisting essentially of a member selected from the group consisting of polyester and polyester/cellulose blends.
A method for transferring electrophoretically separated species from a gel to a membrane, said method comprising passing an electric current across a transfer stack, by imposing a voltage between a pair of electrode plates with said transfer stack between said pair, said transfer stack comprising:
- said gel,
- said membrane immediately adjacent to said gel and in flat surface contact therewith, and
- two sheets of absorbent material wetted with buffer solution, one said sheet immediately adjacent to said gel on the side thereof opposite the side in contact with said membrane and the other said sheet immediately adjacent to said membrane on the side thereof opposite the side in contact with said gel, each said sheet consisting essentially of a member selected from the group consisting of polyester and polyester/cellulose blends
- with one of said electrode plates in direct contact with each of said sheets.
Dependencies are inferred from the claim text.