Acid fungal protease in fermentation of insoluble starch substrates
AssigneeDanisco US Inc.
InventorsGang Duan, Nigel Dunn-Coleman, Oreste Lantero, Craig E. Pilgrim, Jayarama K. Shetty
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Abstract
The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.
Claims11
A method for decreasing the amount of residual starch produced from fermentation of granular starch containing substrates comprising
- fermenting a granular starch containing substrate with a NSP24 protease, a granular starch hydrolyzing enzyme and a fermenting microorganism, wherein the granular starch containing substrate is not cooked, wherein the NSP24 protease has at least 93% amino acid identity to SEQ ID NO:
- 3, and
- obtaining a fermented broth wherein the % residual starch in the broth is decreased compared to the amount of residual starch obtained from a substantially similar method without the protease.
A method of increasing ethanol production in a no cook fermentation comprising contacting a milled insoluble starch substrate with a granular starch hydrolyzing enzyme, an NSP24 protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the substrate to produce ethanol wherein the ethanol production is increased compared to a substantially similar method conducted without the NSP24 protease, and wherein the NSP24 protease has at least 93% amino acid identity to SEQ ID NO:
- 3.
A method of producing ethanol in a no cook simultaneous saccharification and fermentation which includes milled grain substrates comprising adding an NSP24 protease to the simultaneous saccharification and fermentation, wherein the NSP24 protease has at least 93% amino acid identity to SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.