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US 8,071,364 B2Granted
Gene therapy using transposon-based vectors
AssigneesTransGenRx, Inc., Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
InventorsRichard Cooper, Frederick M. Enright, William C. Fioretti
Life of this patent
Estimating the term…
Abstract
Methods and compositions are presented for the administration of transposon based vectors to an animal or human to provide gene therapy to the animal or human.
Claims7
A transposon-based vector comprising an isolated polynucleotide sequence comprising:
- a) a gene operably linked to a first promoter wherein the first promoter is an SV 40 promoter, the gene encoding for a transposase;
- and,
- b) one or more genes of interest encoding for a protein selected from the group consisting of SEQ ID NO:
- 44, 45 and 46, the one or more genes of interest being operably-linked to one or more additional promoters, the one or more genes of interest and their operably-linked promoters are flanked by transposase insertion sequences recognized by the transposase, and the one or more additional promoters are cell-specific promoters or constitutive promoters.
1 more element
An isolated polynucleotide sequence comprising:
- a) one or more genes of interest encoding for a protein selected from the group consisting of SEQ ID NO:
- 44, 45 and 46, the one or more genes of interest being operably-linked to one or more promoters;
- b) a cecropin B poly A nucleotide sequence located 3′ to the one or more genes of interest;
- and,
- c) transposase insertion sequences recognized by a bacterial transposase, the one or more genes of interest and their operably-linked promoters are flanked by the transposase insertion sequences and the one or more additional promoters are cell-specific promoters or constitutive promoters.
2 more elements
Dependencies are inferred from the claim text.