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AssigneeThe United States of America as represented by the Secretary of the Air Force
InventorsLloyd J. Nadeau, Heather R. Luckarift, Jim C. Spain
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A process for the production of ortho-aminophenolic analogs of chloramphenicol using a biocatalyst consisting of pure enzymes, partially purified enzymes, cell lysate, intact cells, or a metal reaction linked with a subsequent enzymatic reaction. The biocatalyst is an enzyme system that makes use of a nitroreductase enzyme that initially reduces the nitroarene to the hydroxylaminoarene and a mutase enzyme that converts the hydroxylaminoarene to an ortho-aminophenol. The biocatalyst can also consist of a coupled, two-step metal and enzyme reaction in which the metal, such as zinc, catalyzes the transformation of the nitroarene to the hydroxylaminoarene and the mutase then catalyzes the transformation of hydroxylaminoarene to the corresponding ortho-aminophenol.
A process for the production of an ortho-aminophenol from a nitroarene comprising reacting a biocatalyst consisting of a nitroreductase enzyme that initially reduces said nitroarene to the corresponding hydroxylaminoarene and a mutase enzyme that converts said hydroxylaminoarene to said ortho-aminophenol, and recovering a fraction containing said ortho-aminophenol, wherein said nitroarene is selected from the group consisting of chloramphenicol, chloramphenicol palmitate, chloramphenicol pantothenate, and mixtures thereof.
A process for the production of an ortho-aminophenol from a nitroarene which comprises reacting said nitroarene with a metal catalyst to obtain the corresponding hydroxylaminoarene and reacting said hydroxylaminoarene with a biocatalyst consisting of a mutase enzyme that converts said hydroxylaminoarene to said ortho-aminophenol, and recovering a fraction containing said ortho-aminophenol wherein said nitroarene is selected from the group consisting of chloramphenicol, chloramphenicol palmitate, chloramphenicol pantothenate, and mixtures thereof.
Dependencies are inferred from the claim text.