# US 8,067,560 B2 · SRSV detection kit

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- Number: US 8,067,560 B2
- Document ID: US8067560B2
- Status: Granted
- Kind: B2
- Application number: 12/498,495
- Filed: 2009-07-07
- Published: 2009-12-10
- Granted: 2011-11-29
- Earliest claimed: 1999-06-22
- Assignees: Japan AS Represented By Director-General of National Institute of Infectious Diseases; Denka Seiken Co., Ltd.
- Inventors: Naokazu Takeda; Katsuro Natori; Tatsuo Miyamura; Kunio Kamata; Toshinori Sato; Seiya Sato
- Web page: https://patentgrep.com/patent/US8067560B2

## CPC

- C07K 16/10: RNA viruses
- C07K 14/005: from viruses
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- G01N 33/56983: {Viruses}
- C12N 2770/16021: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2770/16022: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2770/16023: Virus like particles [VLP]
- G01N 2333/08: RNA viruses
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

This invention relates to an SRSV detection kit comprising all antibodies against SRSV-related virus constituting peptides selected from the following peptide groups (a) to (k), respectively: (a) a peptide having an amino acid sequence represented by SEQ ID NO: 1, and the like, (b) a peptide having an amino acid sequence represented by SEQ ID NO: 2, and the like, (c) a peptide having an amino acid sequence represented by SEQ ID NO: 3, and the like, (d) a peptide having an amino acid sequence represented by SEQ ID NO: 4, and the like, (e) a peptide having an amino acid sequence represented by SEQ ID NO: 5, and the like, (f) a peptide having an amino acid sequence represented by SEQ ID NO: 6, and the like, (g) a peptide having an amino acid sequence represented by SEQ ID NO: 7, and the like, (h) a peptide having an amino acid sequence represented by SEQ ID NO: 8, and the like, (i) a peptide having an amino acid sequence represented by SEQ ID NO: 9, and the like, (j) a peptide having an amino acid sequence represented by SEQ ID NO: 10, and the like, and (k) a peptide having an amino acid sequence represented by SEQ ID NO: 11, and the like. Use of the kit makes it possible to detect most SRSV-related viruses and further to distinguish their serotypes and genogroups, easily and surely.

## Claims

### Claim 1

An isolated polynucleotide comprising a base sequence represented by SEQ ID NO: 21.

### Claim 2 (depends on 1)

A vector, comprising the isolated polynucleotide of claim 1.

### Claim 3 (depends on 2)

A method of preparing a small round structure virus (SRSV) virus-like particle, comprising introducing the vector of claim 2 into insect cells under conditions suitable for expression of a protein encoded by the isolated polynucleotide, wherein the vector is a recombinant baculovirus; and
collecting virus-like particles from the insect cells.