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US 8,067,234 B2Granted
Adipose stromal stem cells for tissue and vascular modification
AssigneeTissue Genesis, Inc.
InventorsKeith L. March, Jalees Rehman
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Abstract
Methods are provided for isolating adipose derived stromal cells from an animal by extracting adipose tissue from the patient, dissecting the tissue, dissociating the tissue into a cell suspension, removing the adipocytes, exposing the cell suspension to red cell lysis buffer, and isolating adipose derived stromal cells.
Claims9
A method for isolating adipose derived stromal cells from a human patient comprising:
- a) performing adipose tissue resection or suction on said patient;
- b) dissecting tissue obtained from said tissue resection or suction and dissociating said tissue into a cell suspension;
- c) removing adipocytes from said cell suspension;
- d) culturing the adipocyte-depleted cell suspension in EGM-2-MV media;
- and
- e) isolating adipose derived stromal cells secreting vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and granulocyte-colony stimulating factor (G-CSF).
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