Optoelectronic detection system

A method of detecting a soluble antigen in a sample comprising the steps of:

• a) combining the sample with:
• i) one or more antibodies that bind to two different epitopes on the soluble antigen;
• and
• ii) an emittor cell comprising an Fc receptor, wherein binding of the Fc receptor to the one or more antibodies results in an increase in intracellular calcium, and wherein said emittor cell further comprises an emittor molecule that emits a photon in response to the increase in intracellular calcium;
• and
• b) measuring photon emission from the emittor cell, thereby detecting the soluble antigen in a sample.

A method of detecting a soluble antigen in a sample comprising the steps of:

• a) combining the sample with a solid substrate comprising a first antibody that binds a first epitope on the soluble antigen, thereby producing a crosslinked soluble antigen bound to a solid substrate;
• b) adding an emittor cell to the crosslinked soluble antigen bound to the solid substrate, wherein said emittor cell comprises a second antibody that binds a second epitope on the soluble antigen, wherein binding of the second antibody to the crosslinked soluble antigen bound to the solid support results in an increase in intracellular calcium, and wherein said emittor cell further comprises an emittor molecule that emits a photon in response to the increase in intracellular calcium;
• and
• c) measuring photon emission from the emittor cell, thereby detecting a soluble antigen in a sample.

A method of detecting a chemical in a sample comprising the steps of:

• a) combining the chemical with a peptide, thereby producing a chemical-peptide complex;
• b) adding an emittor cell comprising one or more antibodies that bind to two different epitopes on the chemical-peptide complex, wherein binding of the one or more antibodies to the chemical-peptide complex results in an increase in intracellular calcium, and wherein said emittor cell further comprises an emittor molecule that emits a photon in response to the increase in intracellular calcium;
• and
• c) measuring photon emission from the emittor cell, thereby detecting a chemical in a sample.

A method of detecting a chemical in a sample comprising the steps of:

• a) combining the chemical with a peptide, thereby producing a chemical-peptide complex;
• b) crosslinking the chemical-peptide complex, thereby producing a crosslinked chemical-peptide complex;
• c) combining with the crosslinked chemical-peptide complex with an emittor cell comprising an antibody that binds to the crosslinked chemical-peptide complex, wherein binding of the antibody to the crosslinked chemical-peptide complex results in an increase in intracellular calcium, and wherein said emittor cell further comprises an emittor molecule that emits a photon in response to the increase in intracellular calcium;
• and
• d) measuring photon emission from the emittor cell, thereby detecting a chemical in a sample.

A method of detecting a chemical in a sample comprising the steps of:

• a) combining the chemical with an antigen-conjugated peptide, thereby producing a chemical-antigen-conjugated peptide complex;
• b) adding an emittor cell comprising one or more antibodies that bind to two different epitopes on the chemical-antigen-conjugated peptide complex, wherein binding of the one or more antibodies to the chemical-antigen-conjugated peptide complex results in an increase in intracellular calcium, and wherein said emittor cell further comprises an emittor molecule that emits a photon in response to the increase in intracellular calcium;
• and
• c) measuring photon emission from the emittor cell, thereby detecting a chemical in a sample.

A method of detecting a chemical in a sample comprising the steps of:

• a) combining the chemical with an antigen-conjugated peptide, thereby producing a chemical-antigen-conjugated peptide complex;
• b) crosslinking the chemical-antigen-conjugated peptide complex, thereby producing a crosslinked chemical-antigen-conjugated peptide complex;
• c) adding an emittor cell comprising an antibody that binds to the crosslinked chemical-antigen-conjugated peptide complex, wherein binding of the antibody to the chemical-antigen-conjugated peptide complex results in an increase in intracellular calcium, and wherein said emittor cell further comprises an emittor molecule that emits a photon in response to the increase in intracellular calcium;
• and
• d) measuring photon emission from the emittor cell, thereby detecting a chemical in a sample.