Device and method for multiple analyte detection

A device comprising:

• a substrate;
• a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet;
• and
• detection reagents pre-loaded in each of the two or more detection chambers;
• wherein the fluid communication comprises a channel and respective fluid connections connecting the channel to the respective detection chambers of the two or more detection chambers, the fluid connections branch off from opposite sides of the channel, the fluid communication comprises a sample delivery channel, and the device further comprises a plurality of dead-end fluid connections, wherein each of the dead-end fluid connections provides a dead-end communication between a respective one of the two or more detection chambers and the sample delivery channel.