Perhydrolases for enzymatic peracid generation
AssigneeE. I. du Pont de Nemous and Company
InventorsRobert DiCosimo, Mark S. Payne, Tyler Yin
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Abstract
Disclosed herein are variants enzymes that are structurally classified as CE-7 enzymes and have perhydrolysis activity. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned variant enzymes as well as methods and compositions comprising the variant enzymes. Further, disinfectant formulations comprising the peroxycarboxylic acids produced by the processes described herein are provided.
Claims9
An isolated polypeptide having perhydrolysis activity and being structurally classified as a carbohydrate esterase family 7 enzyme, said polypeptide having at least 95% amino acid sequence identity to SEQ ID NO:
- 10, provided that a substitution to amino acid 277 of SEQ ID NO:
- 10 is selected from the group consisting of serine, threonine, valine, and alanine, wherein said polypeptide has improved activity for production of an efficacious concentration of percarboxylic acid for disinfection relative to wild-type Thermotoga maritima acetyl xylan esterase of SEQ ID NO:36;
- improved activity across the entire pH range of activity relative to wild-type Thermotoga maritima acetyl xylan esterase of SEQ ID NO:36;
- improved perhydrolysis/hydrolysis ratio relative to wild-type Thermotoga maritima acetyl xylan esterase of SEQ ID NO:36;
- or a combination thereof.
Dependencies are inferred from the claim text.