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US 8,058,504 B2Granted
Cloning of cytochrome P450 genes from Nicotiana
AssigneeU.S. Smokeless Tobacco Company LLC
InventorDongmei Xu
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Abstract
The present invention relates to p450 enzymes and nucleic acid sequences encoding p450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.
Claims35
A method of identifying a Nicotiana plant, comprising the steps of:
- i) hybridizing, under stringent conditions, plant nucleic acid materials from Nicotiana plants to a nucleic acid probe that is 300 nucleotides or more in length, wherein said probe hybridizes to SEQ ID NO:183 under conditions that include a wash in 0.2×SSC at a temperature of 65° C.;
- and
- ii) identifying at least one of said plants that contains a full-length gene that hybridizes to SEQ ID NO:183 under conditions that include a wash in 0.2×SSC at a temperature of 65° C.
A method of identifying a Nicotiana plant, comprising the steps of:
- i) performing PCR on plant nucleic acid materials from Nicotiana plants for the presence of a full-length gene that hybridizes under stringent conditions to SEQ ID NO:
- 183, wherein said stringent conditions include a wash in 0.2×SSC at a temperature of 65° C.;
- and
- ii) identifying at least one of said plants that contains said full-length gene that hybridizes under said stringent conditions to SEQ ID NO:183.
1 more element
A method of identifying a Nicotiana plant, comprising the steps of:
- i) hybridizing plant nucleic acids from Nicotiana plants to a high density oligonucleotide array, said array comprising oligonucleotide probes having nucleotide sequences from a full-length gene that hybridizes to SEQ ID NO:183 under stringent conditions that include a wash in 0.2×SSC at a temperature of 65° C.;
- and
- ii) identifying, based on signal intensity data generated by said hybridization, at least one of said Nicotiana plants that contains said full-length gene.
Dependencies are inferred from the claim text.