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US 8,053,211 B2Granted
Process for the fermentative production of heterologous proteins by means of Escherichia coli
AssigneeWacker Chemie AG
InventorsTobias Dassler, Simone Mitterweger, Guenter Wich
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Abstract
A process for producing a heterologous protein is provided. The process comprises culturing an E. coli strain in a fermentation medium, the E. coli strain harboring a gene which codes for the heterologous protein and is functionally linked to a signal sequence coding for a signal peptide, and releasing the heterologous protein into the fermentation medium, and the heterologous protein being removed from the fermentation medium, wherein the E. coli strain has an attenuated (p)ppGpp-synthetase II activity (PSII activity).
Claims7
A process for producing a heterologous protein, the process comprising:
- culturing an E. coli strain in a fermentation medium including a primary carbon source, the E. coli strain harboring a gene which codes for the heterologous protein and is functionally linked to a signal sequence coding for a signal peptide, the E. coli strain having an attenuated (p)ppGpp-synthetase II activity (PSII activity);
- releasing the heterologous protein into the fermentation medium;
- and
- removing the heterologous protein from the fermentation medium.
1 more element
Dependencies are inferred from the claim text.