Ethanol production in microorganisms
AssigneeJoule Unlimited Technologies, Inc.
InventorsBrian Green, Nikos Reppas, Dan Robertson
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Abstract
The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.
Claims16
An engineered photosynthetic microbe for the biogenic production of ethanol, wherein said engineered cyanobacterium comprises a recombinant pyruvate decarboxylase gene and at least one recombinant alcohol dehydrogenase gene, wherein said recombinant pyruvate decarboxylase gene and at least one recombinant alcohol dehydrogenase gene belong to distinct operons, wherein the copy number of said at least one recombinant alcohol dehydrogenase gene in said cyanobacterium is greater than the copy number of said recombinant pyruvate decarboxylase gene in said cyanobacterium, and wherein the expression of said recombinant alcohol dehydrogenase gene is increased relative to the expression of said pyruvate decarboxylase gene.
Dependencies are inferred from the claim text.