Back to results
US 8,048,628 B2Granted
Methods for nucleic acid amplification on a printed circuit board
AssigneeDuke University
InventorsMichael G. Pollack, Vamsee K. Pamula, Richard B. Fair
Life of this patent
Estimating the term…
Abstract
Methods for amplifying nucleic acid, such as DNA or RNA, on a printed circuit board (PCB) substrate are disclosed. The amplification can be achieved through a variety of methods such as thermocycling or isothermally. The printed circuit board substrate can comprise an array of electrodes for transporting droplets and can be part of a sandwich structure including a top plate parallel to the printed circuit board substrate.
Claims1
A method of amplifying DNA comprising:
- (a) providing a sample solution comprising DNA on a printed circuit board substrate comprising an array of electrodes for transporting droplets on the substrate, and a dielectric coating atop the electrodes, wherein the printed circuit board substrate is provided as part of a sandwich structure, the sandwich structure further comprising:
- (i) a top plate parallel to the printed circuit board substrate and forming a gap between the top plate and the printed circuit board substrate;
- (ii) a seal between the top plate and the printed circuit board substrate;
- and
- (iii) oil in the gap;
- and
- (b) amplifying the DNA on the printed circuit board substrate, wherein the amplifying is achieved by thermocycling wherein the amplifying is accomplished in a droplet in the oil in the gap.
4 more elements
Dependencies are inferred from the claim text.