Compositions and methods for 3-hydroxypropionate bio-production from biomass
AssigneeOPX Biotechnologies, Inc.
InventorMichael D. Lynch
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Abstract
Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate α-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.
Claims26
A recombinant bacterium transformed with at least one exogenous nucleic acid that encodes 3-hydroxypropanoic acid (3-HP) pathway enzymes, wherein said 3-HP pathway enzymes comprise a 2-keto acid decarboxylase and a dehydrogenase, and wherein production of 3-HP is enhanced as compared to a bacterium without the exogenous nucleic acid.
A recombinant bacterium comprising a pathway modified for enhanced production of 3-hydroxypropionic acid (3-HP), wherein said bacterium is transformed with at least one heterologous nucleic acid that encodes enzymes of a 3-HP pathway, said enzymes comprise an oxaloacetate alpha-oxo-decarboxylase and a dehydrogenase, thereby enhancing a 3-HP bio-production process in said bacterium.
A recombinant bacterium comprising a 3-hydroxypropionic acid (3-HP) pathway, wherein said bacterium is transformed with a heterologous nucleic acid that encodes 3-HP pathway enzymes and enhances a 3-HP bio-production process in said bacterium, wherein said 3-HP pathway enzymes comprise an oxaloacetate alpha-oxo-decarboxylase and a dehydrogenase, and wherein the bio-production of 3-HP is performed under microaerobic conditions.
A recombinant Escherichia coli bacterium, comprising a 3-hydroxypropionic acid (3-HP) pathway, wherein said bacterium is transformed with a heterologous nucleic acid that encodes 3-HP pathway enzymes, said 3-HP pathway enzymes comprise a 2-keto acid decarboxylase and a dehydrogenase and wherein production of 3-HP is enhanced as compared to a bacterium without the heterologous nucleic acid.
Dependencies are inferred from the claim text.