Rucola plants with cytoplasmic male sterility (CMS)

A method of producing a male sterile Eruca sativa plant, wherein said plant comprises a male sterile cytoplasm conferring male sterility upon said plant, comprising the steps of:

• a) crossing a cytoplasmic male sterile Brassica oleracea [or a cytoplasmic male sterile Brassica napus] plant comprising Ogura male sterile cytoplasm with an Eruca sativa plant,
• b) rescuing one or more embryos resulting from the cross of step a),
• c) regenerating a plant from one or more embryos of step b) and selecting one or more plant having Eruca sativa phenotype,
• d) doubling the chromosome number of the plant of step c), and
• e) back-crossing one or more plant resulting from step d) with an Eruca sativa plant and selecting for one or more plant with Eruca sativa phenotype.