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US 8,044,260 B2Granted
Method of meristem excision and transformation
AssigneeMonsanto Technology LLC
InventorsErik Dersch, Richard J. Heinzen, Brian Martinell, Anatoly Rivlin, Yuechun Wan, Xudong Ye
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Abstract
The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
Claims26
A high-throughput method for producing transformed cotton plant tissue comprising:
- a) mechanically disrupting cotton seeds to obtain a plurality of cotton embryonic meristem explants;
- b) contacting the explants with a selected DNA sequence encoding a selectable marker to obtain at least a first explant transformed with the selected DNA;
- c) regenerating from at least the first explant transformed with the selected DNA a chimeric cotton plant comprising transgenic plant tissue, wherein regenerating the transgenic cotton plant does not comprise generating a callus culture from the explant;
- d) selecting for transgenic tissue from the chimeric cotton plant by contacting the chimeric cotton plant with a selective agent, wherein the selectable marker confers tolerance to the selective agent;
- and
- e) obtaining a non-chimeric transgenic cotton plant from the transgenic tissue selected in step d).
3 more elements
Dependencies are inferred from the claim text.