Absorbent article containing lateral flow assay device

An absorbent article for receiving urine suspected of containing a leukocyte esterase that is indicative of the presence of urinary tract infection, comprising:

• a substantially liquid impermeable layer;
• a liquid permeable layer;
• an absorbent core positioned between the substantially liquid impermeable layer and the liquid permeable layer;
• and
• a lateral flow assay device positioned between the substantially liquid impermeable layer and the absorbent core such that the device is in fluid communication with urine when provided by a wearer of the article, the lateral flow assay device comprising a chromatographic medium that defines a detection zone, wherein a capture reagent is substantially non-diffusively immobilized within the detection zone that is configured for exhibiting a signal indicative of the presence or absence of the leukocyte esterase in the urine, and wherein the device further comprises a sample application zone that is in fluid communication with the absorbent core and is configured to directly receive the urine therefrom, wherein the detection zone is separate and distinct from the sample application zone and located laterally downstream therefrom.