Chiral indole intermediates and their fluorescent cyanine dyes containing functional groups

A compound of the following general formula (XIV)

• wherein:
• Ra, Rc, Rd, and Rf are independently selected from V or L-V where
• L is a bond or C1-22 straight or branched alkyl, optionally containing 0, 1 or 2 unsaturations or unsaturated pendent or interchain groups selected from alkenyl, alkynyl and aryl groups;
• V is selected from hydrogen, halogen, —OH, —NH2, —SH, —CN, trifluoromethyl, —SO3−, phosphate, phosphonate, quaternary ammonium, —NO2, mono- or di-nitro-substituted benzyl, —COOH, and —NHCORg, where Rg is C1-20 straight or branched alkyl;
• a target bonding group, reactive group, reactive moiety, or NHRh where Rh is H, C1-20 straight or branched alkyl or COOH;
• T is
• wherein each L and V are independently as defined above;
• Y is selected from the group consisting of O, S, —CH═CH—, >C(CH3)2,
• and —N—(CH2)1-10X, wherein X is selected from —COOH, —NH2, —SO3−, —OH and halogen;
• Za and Zb are independently, fused
• and p, u, v and r are independently an integer from 0-4, and at least one V must be —COOH in at least one of T and Y.