P EF-TU expression units
AssigneeBASF SE
InventorsBurkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
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Abstract
The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
Claims22
A method for altering the transcription rate or causing the transcription of a gene in a microorganism compared with the corresponding wild type microorganism comprising
- regulating transcription of a gene in a microorganism by functional linkage to a nucleic acid having promoter activity,
- where the gene is heterologous in relation to the nucleic acid having promoter activity and wherein the nucleic acid having promoter activity comprises
- a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
- 1 or
- b) a nucleic acid molecule comprising a nucleotide sequence having at least 98% identity to the entire nucleotide sequence of SEQ ID NO:
- 1;
- and
- where the microorganism is Corynebacterium glutamicum.
A genetically modified microorganism comprising:
- a nucleic acid having promoter activity which regulates transcription of a gene in the microorganism,
- where the gene is heterologous and functionally linked to the nucleic acid having promoter activity, and
- where the nucleic acid having promoter activity comprises
- a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
- 1 or
- b) a nucleic acid molecule comprising a nucleotide sequence having at least 98% identity to the entire nucleotide sequence of SEQ ID NO:
- 1;
- and
- where the microorganism is Corynebacterium glutamicum.
Dependencies are inferred from the claim text.