Stress tolerant organisms expressing a map kinase homologue
AssigneeCarmel-Haifa University Economic Corp. Ltd.
InventorsEviatar Nevo, Yan Jin, Weining Song
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Abstract
The present invention provides a MAP kinase homologue gene, designated EhHOG, isolated from Eurotium herbariorum, a common fungal species from the extreme hypersaline environment of the Dead Sea, capable of improving tolerance of plants and other organisms to abiotic stresses such as osmotic, heat, dehydration, freezing-thawing, oxidative and salinity stress.
Claims16
An isolated nucleic acid, comprising a DNA having a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 1 and isolated DNA having a nucleotide sequence differing from SEQ. ID NO:
- 1 in codon sequence due to the degeneracy of the genetic code.
A chimeric construct capable of expression in host cells, comprising:
- (a) a DNA sequence of SEQ ID NO:
- 1 coding for the EhHOG protein of SEQ ID NO:
- 2, and (b) DNA sequences enabling expression of the EhHOG protein in host cells.
A transgenic plant transformed with the DNA of SEQ ID NO:
- 1 that encodes the protein EhHOG consisting of the amino acid sequence as shown in SEQ ID NO:
- 2, said DNA being operably linked to DNA sequences enabling expression of the EhHOG protein in plant cells and subsequent improvement of tolerance of the plant to abiotic stress.
A biologically pure culture of a microorganism transformed with an isolated nucleic acid comprising the DNA of SEQ ID NO:
- 1 that encodes the protein EhHOG consisting of the amino acid sequence as shown in SEQ ID NO:
- 2, said DNA being operably linked to DNA sequences enabling expression of the EhHOG protein in the microorganism cells and subsequent improvement of tolerance of the microorganism to abiotic stress.
Dependencies are inferred from the claim text.