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AssigneeWako Pure Chemical Industries, Ltd.
InventorTomokazu Ishikawa
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An object of the present invention is to provide a novel primer and prove for detecting tubercle bacillus capable of avoiding false positive, and an easy-to-use, rapid and high-sensitivity method for detecting human tubercle bacillus (Mycobacterium tuberculosis) using the same. The present invention relates to an oligonucleotide comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis, a primer and a probe containing the oligonucleotide for detecting Mycobacterium tuberculosis, and a method for detecting Mycobacterium tuberculosis using the primer and the probe.
An isolated or purified oligonucleotide consisting of a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, or SEQ ID NO:8, or the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis.
A primer for detecting Mycobacterium tuberculosis comprising a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5, or the complementary sequence thereof, wherein the primer has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis.
A probe for detecting Mycobacterium tuberculosis comprising a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:11, or the complementary sequence thereof, wherein the probe has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis.
A kit for detecting Mycobacterium tuberculosis comprising:
Dependencies are inferred from the claim text.