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US 8,044,183 B2Granted
Process for the production of immunogenic compositions
AssigneeGlaxosmithkline Biologicals SA
InventorsTeresa Cabezon Silva, Joseph Cohen, Moncef Mohamed Slaoui, Carlota Vinals Bassols
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Abstract
The present invention relates to a process for producing immunogenic polypeptides, comprising reducing disulfide bonds and blocking the resulting free thiol group with a blocking agent. The immunogenic peptides comprise a fragment of MAGE A3.
Claims12
A process for the production of an immunogenic polypeptide comprising the steps of:
- (i) treating a polypeptide consisting of SEQ ID NO:2 to reduce a disulfide bond and produce a free thiol group;
- (ii) blocking said free thiol group with a blocking agent;
- (iii) purifying a polypeptide resulting from step (ii) using one or more chromatographic purification steps,
- where the purified polypeptide is immunogenic and contains said blocking agent.
1 more element
A process for the production of an immunogenic composition comprising the steps of:
- (i) treating a polypeptide consisting of SEQ ID NO:2 to reduce a disulfide bond and produce a free thiol group;
- (ii) blocking said free thiol group with a blocking agent;
- (iii) purifying a polypeptide resulting from step (ii) using one or more chromatographic purification steps, where the purified polypeptide is immunogenic and contains said blocking agent;
- and
- (iv) formulating the purified polypeptide as a pharmaceutical composition.
2 more elements
Dependencies are inferred from the claim text.