Cell penetrating peptide
AssigneeMitsubishi Tanabe Pharma Corporation
InventorsShusei Uno, Kaeko Kamide, Hiroshi Nakakubo
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Abstract
According to the present invention, it is possible to provide a novel cell penetrating peptide that transports proteins into cells and/or into nuclei at higher frequency than conventional cell penetrating peptides, and a pharmaceutical containing the peptide.
Claims31
An isolated peptide having the capability of translocation to cells, wherein said peptide comprises the amino acid sequence shown below:
- B-X-Z-X-Arg-Z-Tyr-J-X-O1-X-Arg-O2-X-X or X-X-O1-Arg-X-O2-X-J-Tyr-Z-Arg-X-Z-X-B
- wherein
- (i) B is arginine or lysine,
- (ii) at least one of O1 or O2 is arginine, wherein, when one of O1 or O2 is arginine, the remaining O1 or O2 is an arbitrarily chosen amino acid,
- (iii) Z is a hydrophobic amino acid,
- (iv) J is serine or alanine, and
- (v) X is an arbitrarily chosen amino acid;
- with the proviso that when Z is leucine and O1 is arginine, then J is alanine or B is lysine.
An isolated peptide having the capability of translocation to cells which is
- (a) a peptide comprising the amino acid sequence of any one of SEQ ID NOS:
- 1, 2, 6-13, 15, 17-20, 22-30, 32 and 33 or the reversed-chain peptide thereof;
- or
- (b) a peptide comprising the amino acid sequence of (a) above wherein one or two amino acids have been substituted, deleted, added or inserted.
An isolated peptide having the capability of translocation to cells, wherein said peptide is one of the following (a) or (b):
- (a) a peptide comprising the amino acid sequence of any one of SEQ ID NOS:
- 3-5, 14, 16, 21, 31 and 35-47 or the reversed-chain peptide thereof;
- (b) a peptide comprising the amino acid sequence of (a) above wherein one or two amino acids have been substituted, deleted, added or inserted.
An isolated peptide comprising the amino acid sequence of any one of SEQ ID NOS:
- 1 to 33 and 35-47 or the reversed-chain peptide thereof.
An isolated D-form peptide having the capability of translocation to cells, wherein said peptide comprises the amino acid sequence shown below:
- (a) B-X-Z-X-Arg-Z-Tyr-J-X-O1-X-Arg-O2-X-X or X-X-O1-Arg-X-O2-X-J-Tyr-Z-Arg-X-Z-X-B
- wherein
- (i) B is arginine or lysine,
- (ii) at least one of O1 or O2 is arginine, wherein, when one of O1 or O2 is arginine, the remaining O1 or O2 is an arbitrarily chosen amino acid,
- (iii) Z is a hydrophobic amino acid,
- (iv) J is serine or alanine, and
- (v) X is an arbitrarily chosen amino acid;
- or
- (b) the amino acid sequence of (a) above wherein one or two amino acids have been substituted, deleted, added or inserted.
A D-form of an isolated peptide that comprises any one of SEQ ID NOS:
- 1-33 or 35-47 or a reversed chain peptide thereof.
Dependencies are inferred from the claim text.