Methods for molecular detection
AssigneeBiotex, Inc.
InventorsGeorge Jackson, Christopher Quan
Life of this patent
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Abstract
This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.
Claims20
A method for molecular detection comprising:
- contacting at least one molecular probe comprising a nucleic acid and at least one reporter molecule comprising a nucleic acid with a sample for detecting the presence of a target in said sample, said at least one molecular probe being substantially complementary to at least a portion of said reporter molecule and having binding affinity for said target;
- and
- performing a quantitative or semi-quantitative amplification reaction on the at least one reporter molecule;
- wherein said at least one molecular probe binds to at least one conjugate region of said at least one reporter molecule in the absence of the target and enables amplification of said at least one reporter molecule.
, A method for molecular detection comprising:
- contacting at least one molecular probe comprising a nucleic acid and at least one reporter molecule comprising a circular nucleic acid with a sample for detecting the presence of a target in said sample, said at least one molecular probe having binding affinity for said target;
- and
- performing a quantitative or semi-quantitative amplification reaction on said at least one reporter molecule;
- wherein said at least one molecular probe binds to at least one conjugate region of said at least one reporter molecule in the absence of the target and enables amplification of said at least one reporter molecule.
A method for molecular detection comprising:
- contacting at least one molecular probe comprising a nucleic acid and at least one reporter molecule comprising a nucleic acid with a sample for detecting the presence of a target in said sample, said at least one molecular probe being substantially complementary to at least a portion of said reporter molecule and having competitive binding affinities for said target and said reporter molecule;
- and
- performing a quantitative or semi-quantitative amplification reaction on said at least one reporter molecule;
- wherein said at least one molecular probe binds to at least one conjugate region of said at least one reporter molecule in absence of said target and enables amplification of said at least one reporter molecule.
Dependencies are inferred from the claim text.