Method for precipitating mono and multiple layers of organophosphoric and organophosphonic acids and the salts thereof in addition to use thereof

A method which comprises precipitating mono or multiple layers of a mixture of at least two different acids selected from the group consisting of organophosphoric acids of the general formula I (A)

• Y—B—OPO3H
• or of organophosphonic acids of the general formula I (B)
• Y—B—PO3H2
• and the salts thereof, on a substrate surface of pure or mixed oxides nitrides or carbides of metals or semiconductors,
• wherein B is an alkyl, alkenyl, alkinyl, aryl, aralkyl, hetaryl or hetarylalkyl residue and Y is hydrogen or a functional group from the hydroxy, carboxy, amino, optionally low-alkyl-substituted mono or dialkylamino series, thiol, or a negative acid group from the ester, phosphate, phosphonate, sulfate, sulfonate, maleimide, succinimydyl, epoxy, acrylate series, wherein a biologically effective recognition element is coupled to B or Y by addition or substitution reaction,
• wherein compounds may also be added conferring on the substrate surface a resistance to protein adsorption and/or to cell adhesion and the B chain may optionally be comprised of one or more ethylene oxide groups, rather than one or more —CH2- groups, and wherein the organophosphoric acid or organophosphonic acid is precipitated from water-soluble salts of a compound of formula (IA) or (IB) for the treatment of these surfaces, of sensor platforms, implants and medical accessory devices, and wherein the biologically effective recognition element comprises peptides, proteins, glycoproteins, growth factor, such as TGF-β, or BMP (bone morphogenic protein).

A method which comprises precipitating mono or multiple layers of a mixture of at least two different acids selected from the group consisting of organophosphoric acids of the general formula I (A)

• Y—B—OPO3H
• or of organophosphonic acids of the general formula I (B)
• Y—B—PO3H2
• and the salts thereof, on a substrate surface of pure or mixed oxides, nitrides or carbides of metals or semiconductors,
• wherein B is an alkyl, alkenyl, alkinyl, aryl, aralkyl, hetaryl or hetarylalkyl residue and Y is hydrogen or a functional group from the hydroxy, carboxy, amino, optionally low-alkyl-substituted mono or dialkylamino series, thiol, or a negative acid group from the ester, phosphate, phosphonate, sulfate, sulfonate, maleimide, succinimydyl, epoxy, acrylate series, wherein a biological, biochemical or synthetic indicator element may be coupled to B or Y by addition or substitution reaction, wherein compounds are also added conferring on the substrate surface a resistance to protein adsorption and/or to cell adhesion and the B chain may optionally be comprised of one or more ethylene oxide groups, rather than one or more —CH2- groups, and wherein the organophosphoric acid or organophosphonic acid is precipitated from water-soluble salts of a compound of formula (IA) or (IB) for the treatment of these surfaces, of sensor platforms, implants and medical accessory devices, and wherein the compounds which confer on the substrate surface a resistance to protein adsorption and/or to cell adhesion are selected from the group of compounds which are formed from oligo(ethylene oxide), phosphoryl choline, heparin, saccharides, albumins, especially bovine serum albumin or human serum albumin, casein, nonspecific, polyclonal or monoclonal, heterologous or for the analyte or analytes to be determined empirically nonspecific antibodies (especially for immunoassays), detergents (such as Tween 20), fragmented natural DNA or synthetic DNA not hybridizing with polynucleotides for analysis, such as a herring or salmon sperm extract (especially for polynucleotide hybridization assays), or also uncharged, but hydrophilic polymers, such as polyethylene glycols or dextrans.

A method which comprises precipitating mono or multiple layers of a mixture of at least two different acids selected from the group consisting of organophosphoric acids of the general formula I (A)

• Y—B—OPO3H
• or of organophosphonic acids of the general formula I (B)
• Y—B—PO3H2
• and the salts thereof, on a substrate surface of pure or mixed oxides, nitrides or carbides of metals or semiconductors,
• wherein B is an alkyl, alkenyl, alkinyl, aryl, aralkyl, hetaryl or hetarylalkyl residue and Y is hydrogen or a functional group from the hydroxy, carboxy, amino, optionally low-alkyl-substituted mono or dialkylamino series, thiol, or a negative acid group from the ester, phosphate, phosphonate, sulfate, sulfonate, maleimide, succinimydyl, epoxy, acrylate series, wherein a biological, biochemical or synthetic recognition element is coupled to B or Y by addition or substitution reaction, wherein compounds are, also added conferring on the substrate surface a resistance to protein adsorption and/or to cell adhesion in a SAM molecule and the B chain may optionally be comprised of one or more ethylene oxide groups, rather than one or more —CH2- groups, and wherein the organophosphoric acid or organophosphonic acid is precipitated from water-soluble salts of a compound of formula (IA) or (IB) for the treatment of these surfaces, of sensor platforms, implants and medical accessory devices.